4i refines cell sample analysis

IF Imaging

Researchers at the University of Zurich have developed a novel method for analysing cells and their components called Iterative Indirect Immunofluorescence Imaging (4i).

4i makes it possible to observe the spatial distribution of at least 40 proteins and their modifications in the same cell for hundreds of thousands of cells simultaneously at various levels, from the tissue down to the organelle level.

“4i is the first imaging technique which gives us a multiplexed tissue-to-organelle view of biological samples. We can link multiplexed information derived at the tissue, cellular and subcellular level in one and the same experiment,” says lead author Gabriele Gut.

Immunofluorescence (IF) uses antibodies to visualise and locate proteins in biological samples. While the standard IF method usually marks three proteins, 4i uses off-the-shelf antibodies and conventional fluorescence microscopes to visualise 10 times more proteins by iterative hybridisation and removal of antibodies from the sample.

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4i refines cell sample analysis

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