"To speak of Gaddafi's resignation is a humiliation for all Libyans," she said in a brief statement at her father's Tripoli residence before hundreds of young supporters.
"You want to kill my father, pretending to protect civilians," she said, referring to Western air raids. "Where are these civilians? Are they carrying machine guns, RPGs, hand grenades?"
Aisha Gaddafi is a law professor who was a member of executed Iraqi leader Saddam Hussein's defence team following his ouster by US-led forces in 2003.
Britain, France and the US yesterday said that a Libyan future including Gaddafi was "unthinkable" and would represent an "unconscionable betrayal" by the rest of the world.
British Prime Minister David Cameron, France's President Nicolas Sarkozy and US President Barack Obama vowed they would "not rest until the UN... resolutions have been implemented", in a joint article published in several international newspapers.
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