The unseeded Indian had the advantage after winning the first set but the 17th seed Souza on Tuesday shut the door on him with a 4-6 6-2 8-6 triumph in the opening round of the clay court Major's qualifying event.Amritraj's first and only appearance in a Grand Slam main draw was way back in 2002 when he entered the US Open as a wild card.
Now Indian hopes rest on Somdev Devvarman, who has advanced to the second round after beating Jiri Vanek.Somdev will take on Kiwi Daniel King-Turner.
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